Andrology Laboratory Techniques
	How to set up Andrology Laboratory and what are the equipment required?
	Handling, cleaning, maintenance and calibration of different equipment-Laminar Flow workstation, Microscopes, Centrifuges, etc.
	Comprehensive semen analysis-Macroscopic and Microscopic-density, motility, viability, round cell differentiation etc.
	Sperm morphology assessment according to WHO and Strict (Kruger) criteria.
	Sperm function tests-Hyaluronon binding assay, Sperm-DNA fragmentation test, Sperm Mar test, Sperm survival test.
	Semen cryopreservation-both neat and processed sample.
	Semen preparation for IUI-Classical method, Standard method and Density gradient method.
Embryology Laboratory Techniques
	How to set up Embryology laboratory-Lab space and layout, equipment, design flooring, HEPA filter etc.
	Monitoring, calibration and maintenance of equipment-measuring and adjustment of carbon dioxide (CO2) & temperature of incubators and cleaning of incubators
	Embryology lab maintenance-daily, weekly and monthly.
	List and selection of disposables and culture media.
	Preparation of Embryology Lab for oocyte retrieval the day before-checklist.
	Preparation of lab on the day of oocyte retrieval
	Identification of granulose cells, cumulus and corona cells and oocytes
	Separation of oocytes and further culture
	Preparation of semen for insemination of oocytes-processing of ejaculated semen
	Method of oocyte insemination-short co-incubation, etc
	Dissection of oocytes and fertilization check
	Criteria for fertilization check
	Day – 1 Fertilization check, classification of zygotes and further culture
	Evaluation of different zygote morphology using digital pictures
	Day 2 – Assessment of embryo cleavage and identification of good embryos
	Day 3 – Assessment of cleavage and selection of best quality embryos for transfer
	Day 5/6- Blastocyst culture -technique
Learning Material
	Hands-on practical one to one using unlimited number of mouse oocytes. Videos, Lectures.
Embryo Transfer
	Preparation for embryo transfer
	Selection of embryos for transfer- day 2, day3 or day5
	Technique of embryo loading into the catheter (mock transfer)
Micromanipulation (practical hands-on)
Intracytoplasmic sperm injection (ICSI)
	Familiarization and operation of equipment (Training in the use of various brands of microscopes, Micromanipulators, micromanipulation tools, media etc).
	Setting up and alignment of micromanipulators and tools at different angles - Technique and tips.
	Preparation of oocytes for micro-injection-use of appropriate pipettes for dissection of oocytes in hyaluronidase and use of appropriate media.
	Preparation of sperm and egg dishes for micromanipulation.
	Selection and method of picking sperm-Technique and tips.
	Technique of microinjection-Tips.
Learning Material
	Hands-on practical one to one using unlimited number of mouse oocytes.
Assisted Hatching Chemical and laser Method of hatching
	Selection of embryos for hatching
	Preparation of Acid Tyrode prior to assisted hatching
	Preparation of dishes for hatching
	Technique of hatching and tips
	Setting up of laser for hatching
	Technique and precautions
Learning Material
	Hands-on practical one to one using unlimited number of mouse embryos
Embryo Cryopreservation: Slow Freezing and Thawing Method
	Familiarization and operation of freezing machines
	Selection of straws, plugs, canes, visitubes etc
	Selection of culture media
	Preparation for embryo freezing
	Selection of embryos for freezing
	Technique of embryo freezing, loading of embryos
	Running the freezing machine
	Storage of embryos
Embryo thawing method
	Hands-on practical one to one using unlimited number of mouse embryos
	Assessment of post-thaw survival
Ultra-rapid Freezing Method-Vitrification
	Selection of Carriers for vitrification
	Selection of vitrification solutions
	Technique and precautions
	Warming of vitrified embryos
	Post-thaw assessment
Learning Material
	Hands-on practical one to one using unlimited number of mouse embryos